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KMID : 0857020100250010031
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Kosin Medical Journal
2010 Volume.25 No. 1 p.31 ~ p.34
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Seizures and Behavioral Changes Induced by Homocrysteine In Rats
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김광수:Kim Kwang-Soo
허소영:Huh So-Young
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Abstract
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Background: Homocysteine is epileptogenic and can induce seizures in immature and adult animals. Homocysteine and
its derivatives are structurally similar to glutamate and express a direct excitatory effect on N-methyl-D-aspartate
(NMDA) receptors. The aim of this study is to characterize the convulsant effect of homocysteine after intraperitoneal
administration of homocysteine in rats.
Methods: Twenty-four Sprague-Dawley male rats weighing 120-145 g were treated with single intraperitoneal injection
of D,L-homocysteine (11 mmol kg). The animals were observed for 90 min after injection. The incidence, latencies,
pattern of motor seizures, and behavioral phenomena were noted. Seizure severity was determined by a rating scale
with grades defined as: 1-head nodding, lower jaw twitching; 2-myoclonic body jerks, bilateral forelimb clonus with
full rearing; 3-progression to generalized clonic convulsion followed by tonic extension of fore- and hind limbs and
tail; 4-prolonged tonic-clonic convulsion lasting over 10 seconds.
Results: All rats sat motionless for some minutes after the injection of homocysteine, and then washing, sniffing,
shuffling, and chewing appeared. The seizure episodes of grade 1 were appeared in 91.7%, grade 2 in 87.5%, grade
3 in 37.5%, and grade 4 in 8.3%. The latencies of grade 1, 2, and 3 seizure episodes were 15.3 ± 9.6 min, 30.9 ±
13.3 min, and 58.9 ± 14.8 min respectively. The seizure episodes of grade 4 were appeared 28 min and 64 min after
homocysteine injection in 2 rats.
Conclusion: These results show that homocysteine administration elicits seizures and behavioral responses, and suggests a possible animal model for studying epilepsy.
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KEYWORD
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Behavioral changes, Homocysteine, Rat, Seizures
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